Activator protein 2 (AP-2/TFAP2A) Polyclonal Antibody (CAB21727)
The Activator Protein 2 (AP-2) / TFAP2A Polyclonal Antibody is a powerful tool for researchers studying the activity of the AP-2 transcription factor TFAP2A. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the AP-2 protein, this antibody allows for the detection and analysis of TFAP2A in a variety of cell types, making it an essential tool for investigations in molecular biology and cancer research.TFAP2A is a key transcription factor that regulates the expression of genes involved in cell proliferation, differentiation, and apoptosis.
Its role in controlling cellular processes makes it a critical target for understanding various diseases, including cancer. By utilizing the AP-2/TFAP2A Polyclonal Antibody, researchers can gain valuable insights into the mechanisms by which TFAP2A functions, potentially leading to new therapeutic strategies for treating cancer and other diseases impacted by dysregulated gene expression.
Product Name:
Activator protein 2 (AP-2/TFAP2A) Polyclonal Antibody
SKU:
CAB21727
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Activator protein 2 (AP-2/TFAP2A) (NP_003211.1).
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha; Activator protein 2 (AP-2/TFAP2A)
Positive Sample:
HeLa,C6
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
48kDa
Observed MW:
50kDa
The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7020
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Activator protein 2 (AP-2/TFAP2A) antibody (CAB21727) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
