β-Actin Rabbit Polyclonal Antibody (CABC006)
- SKU:
- CABC006
Frequently bought together:
Description
| Product Name: | β-Actin Rabbit Polyclonal Antibody |
| SKU: | CABC006 |
| Size: | 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human β-Actin (NP_001092.1). |
| Sequence: | MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF |
| Tested Applications: | WB |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | BRWS1; PS1TP5BP1; β-Actin |
| Positive Sample: | HeLa,MCF7,A-431,NIH/3T3,C6,Mouse testis,Mouse kidney,Rat testis |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, cytoskeleton. |
| Calculated MW: | 42kDa |
| Observed MW: | 42kDa |
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
| Purification Method: | Affinity purification |
| Gene ID: | 60 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using β-Actin Rabbit pAb (AC006) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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