The Actin Antibody (PAC017454) is a high-quality research tool designed for the detection and analysis of actin, a critical protein involved in maintaining cell structure and movement. This polyclonal antibody, produced in rabbits, is highly specific and sensitive for detecting actin in human samples. It has been validated for use in Western blot applications, providing researchers with a reliable tool for studying actin in various cell types.Actin is essential for numerous cellular processes, including cell division, movement, and signaling. Dysregulation of actin dynamics has been implicated in a variety of diseases, including cancer, cardiovascular disorders, and neurological conditions.
By using the Actin Antibody, researchers can explore the role of actin in these disease contexts and potentially identify new therapeutic targets for intervention.Overall, the Actin Antibody is a valuable tool for researchers conducting studies in cell biology, cancer research, and other fields where understanding actin dynamics is crucial. Its high specificity and sensitivity make it an ideal choice for detecting actin in a variety of experimental settings.
Antibody Name:
ACTIN Antibody (PACO17454)
Antibody SKU:
PACO17454
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:500-1:2000, WB:1:500-1:2000
Species Reactivity:
Plants
Immunogen:
Synthetic peptide of Arabidopsis thaliana actin
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 10%SDS-PAGE, Lysate: 50 μg, Lane 1-7: Cinnamomum camphora tissue, Nicotiana tabacum tissue, Triticum aestivuml, Arabidopsis thaliana leaves, Brassica campestris flowers, Brassica campestris, Vicit Faba leaves, Primary antibody: PACO17454(ACTIN Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds.
Background:
Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.
Synonyms:
Actin
UniProt Protein Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the reproductive actins.
UniProt Protein Details:
NCBI Summary:
Encodes one of eight Arabidopsis actins. ACT4 belongs to the reproductive actin subclass which is predominantly expressed in developing and reproductive tissues, such as pollen, pollen tubes, ovules, and developing seeds. Expression of the ACT4/GUS fusion was restricted to young vascular tissues, tapetum, and developing and mature pollen.
