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Anti-ACTB Antibody (MACO0098)

SKU:
MACO0098
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
NHP - Monkey
Canine
Host Species:
Mouse
Isotype:
IgG
Applications:
ELISA
WB
Antibody Type:
Monoclonal Antibody
Conjugation:
Unconjugated
€269
Frequently bought together:

Description

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Anti-ACTB Antibody (MACO0098)

The ACTB Monoclonal Antibody (MACO0098) is a powerful tool for researchers studying actin, a crucial protein involved in cell structure and movement. This monoclonal antibody, derived from mouse hybridoma cells, is highly specific to the ACTB protein and is suitable for various applications, including Western blotting and immunofluorescence.Actin is a key component of the cytoskeleton and is involved in various cellular processes such as cell division, cell adhesion, and intracellular transport. The ACTB Monoclonal Antibody recognizes the beta isoform of actin, allowing for precise detection and analysis of actin expression in different cell types and tissues.

By targeting ACTB, researchers can gain insight into the mechanisms underlying cellular function and behavior. This antibody is particularly valuable for studies in cell biology, cancer research, and developmental biology, where actin dynamics play a crucial role. With its high specificity and sensitivity, the ACTB Monoclonal Antibody (MACO0098) is a valuable tool for elucidating the functions of actin in various biological processes.

Product Name:Anti-ACTB Antibody
Product Sku:MACO0098
Size:50ug
Host Species:Mouse
Tested Applications:ELISA, WB
Recommended Dilutions:WB:1:500-1:5000
Species Reactivity:Human,Mouse,Rat,Mk,Dg
Immunogen:Synthetic Peptide
Form:Liquid
Storage Buffer:PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Purification Method:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clonality:Monoclonal
Isotype:IgG
Conjugate:Non-conjugated
Background:
Synonyms:ACTB; Actin, cytoplasmic 1; Beta-actin
Product ImageWestern blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, diluted at 1:5000.
UniProt Protein Function:Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
UniProt Protein Details:

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Ref.11 Ref.12 Ref.15 Ref.22

Subcellular location: Cytoplasm › cytoskeleton. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.17

Post-translational modification: ISGylated. Ref.13Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced

By similarity.

Involvement in Disease: Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [

MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Ref.23Defects in ACTB are the cause of Baraitser-Winter syndrome type 1 (BRWS1) [

MIM:243310]. A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. Ref.25

Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Sequence similarities: Belongs to the actin family.

NCBI Summary:This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
UniProt Code:P60709
NCBI GenInfo Identifier:46397333
NCBI Gene ID:60
NCBI Accession:P60709.1
UniProt Secondary Accession:P60709,P02570, P70514, P99021, Q11211, Q64316, Q75MN2 Q96B34, Q96HG5,
UniProt Related Accession:P60709
Molecular Weight:67 KD
NCBI Full Name:Actin, cytoplasmic 1
NCBI Synonym Full Names:actin, beta
NCBI Official Symbol:ACTB  
NCBI Official Synonym Symbols:BRWS1; PS1TP5BP1  
NCBI Protein Information:actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name:Actin, cytoplasmic 1
UniProt Synonym Protein Names:Beta-actin
Protein Family:Actin
UniProt Gene Name:ACTB  
UniProt Entry Name:ACTB_HUMAN

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