The ACTA2 Polyclonal Antibody (CAB1011) is a valuable tool for researchers studying the ACTA2 gene, which encodes for smooth muscle alpha-actin, a protein involved in smooth muscle contraction and cell motility. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications.ACTA2 is an essential component of smooth muscle cells, playing a crucial role in various physiological processes such as vascular contraction, organ development, and wound healing. Dysregulation of ACTA2 expression has been linked to diseases like thoracic aortic aneurysms and dissections, as well as smooth muscle cell tumors.
By utilizing the ACTA2 Polyclonal Antibody, researchers can effectively detect and analyze the expression levels of ACTA2 in different cell types, enabling a better understanding of its function in normal and diseased states. This antibody is an invaluable tool for studies in cardiovascular biology, smooth muscle-related disorders, and developmental biology, ultimately contributing to the advancement of medical research and potential therapeutic interventions.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
Purification Method:
Affinity purification
Gene ID:
59
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (CAB1011) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
