Acta1 Antibody (PACO26417)
- SKU:
- PACO26417
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Fish - Zebrafish
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Acta1 Antibody (PACO26417)
The ACTA1 Monoclonal Antibody (PAC026417) is a specific antibody designed for research involving ACTA1, a gene encoding a muscle protein involved in muscle contraction. This antibody, created using hybridoma technology, is highly sensitive and specific, making it ideal for detecting and analyzing ACTA1 expression in various biological samples. ACTA1, also known as alpha-actin 1, is a crucial component of sarcomeres in muscle cells and plays a key role in muscle function. Mutations in the ACTA1 gene have been associated with skeletal muscle disorders, making it a valuable target for research into muscle-related diseases.
The ACTA1 antibody can be used in applications such as Western blotting, immunofluorescence, and immunohistochemistry to study the expression and localization of ACTA1 in different tissues. By understanding the expression and function of ACTA1, researchers can gain insights into muscle development, function, and disease pathology. This antibody provides a reliable tool for investigating the role of ACTA1 in muscle biology and can contribute to advancements in the field of muscle research and therapy development.
| Antibody Name: | Acta1 Antibody (PACO26417) |
| Antibody SKU: | PACO26417 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Mouse, Rat, Zebrafish |
| Immunogen: | Mouse Actin, α skeletal muscle (Native Protein) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: Acta1 antibody at 2µg/ml Lane 1: Mouse brain tissue Lane 2: Mouse lung tissue Lane 3: Rat muscle tissue Lane 4: Zebrafish lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa . |
| Background: | Actin is a globular, roughly 42-kDa moonlighting protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100μM. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular processes including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes. In vertebrates, three main groups of actin isoforms, alpha, beta, and γ have been identified. The α actins are found in muscle tissues and are a major constituent of the contractile apparatus. The β and γ actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. |
| Synonyms: | Actin, α skeletal muscle (Alpha-actin-1) [Cleaved into: Actin, α skeletal muscle, intermediate form], Acta1, Acta |
| UniProt Protein Function: | ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Contractile; CytoskeletalChromosomal Location of Human Ortholog: 1q42.13Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeletonMolecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP bindingBiological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to steroid hormone stimulus; response to mechanical stimulus; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament slidingDisease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion |
| UniProt Protein Details: | |
| NCBI Summary: | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause a variety of myopathies, including nemaline myopathy, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects with manifestations such as hypotonia. [provided by RefSeq, Sep 2019] |
| UniProt Code: | P68134 |
| NCBI GenInfo Identifier: | 4501881 |
| NCBI Gene ID: | 58 |
| NCBI Accession: | NP_001091.1 |
| UniProt Secondary Accession: | P68134,P68136, P68135, P68137, P68134, P68139, P68138 P04751, P68035, P68033 |
| UniProt Related Accession: | P68134,P68133 |
| Molecular Weight: | Calculated MW:H=42M=42Rat=42 KDa |
| NCBI Full Name: | actin, alpha skeletal muscle |
| NCBI Synonym Full Names: | actin alpha 1, skeletal muscle |
| NCBI Official Symbol: | ACTA1Â Â |
| NCBI Official Synonym Symbols: | ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDMÂ Â |
| NCBI Protein Information: | actin, alpha skeletal muscle |
| UniProt Protein Name: | Actin, alpha skeletal muscle |
| UniProt Synonym Protein Names: | Alpha-actin-1 |
| Protein Family: | |
| UniProt Gene Name: | ACTA1Â Â |
| UniProt Entry Name: | ACTS_HUMAN |
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