The ACSS2 Polyclonal Antibody (CAB6472) is a valuable tool for research involving ACSS2, an enzyme involved in acetate metabolism and lipid synthesis pathways. The antibody, produced in rabbits, is highly specific and reacts with human samples, making it suitable for use in various experimental techniques such as Western blotting.ACSS2 plays a key role in cellular metabolism, particularly in cancer cells where it is upregulated to support rapid growth and proliferation. By targeting ACSS2, researchers can gain insights into the mechanisms underlying cancer cell metabolism and potentially identify new therapeutic targets for cancer treatment.
This antibody enables the detection and analysis of ACSS2 protein levels in different cell types, making it an essential tool for studies in cancer biology, metabolic disorders, and lipid metabolism. Understanding the function of ACSS2 is critical for developing targeted therapies that exploit metabolic vulnerabilities in cancer cells.
Product Name:
ACSS2 Rabbit Polyclonal Antibody
SKU:
CAB6472
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 462-701 of human ACSS2 (NP_061147.1).
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55902
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACSS2 Rabbit pAb (CAB6472) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
