The ACSL5 Polyclonal Antibody (CAB1270) is a valuable tool for research involving Acyl-CoA synthetase long-chain family member 5 (ACSL5), an enzyme involved in fatty acid metabolism. This antibody, produced in rabbits, is highly specific to human ACSL5 samples and has been validated for use in various applications, including Western blotting.ACSL5 is a key player in lipid metabolism, particularly in the activation of long-chain fatty acids for energy production and lipid synthesis. Dysregulation of ACSL5 has been linked to metabolic disorders, such as obesity and diabetes, making it an important target for research in the fields of nutrition, metabolism, and metabolic diseases.
By using the ACSL5 Polyclonal Antibody, researchers can detect and analyze the expression of ACSL5 in different cell types and tissues, providing valuable insights into its function and regulation. This antibody is an essential tool for studies aiming to understand the role of ACSL5 in metabolic pathways and its potential implications for human health.
Product Name:
ACSL5 Rabbit Polyclonal Antibody
SKU:
CAB1270
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 500-739 of human ACSL5 (NP_057318.2).
Endoplasmic reticulum, Endoplasmic reticulum membrane, Mitochondrion, Mitochondrion outer membrane, Single-pass type III membrane protein.
Calculated MW:
76kDa
Observed MW:
70-76kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
51703
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ACSL5 antibody (CAB1270) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
