The ACSL4 Polyclonal Antibody (CAB6826) is a valuable tool for researchers studying ACSL4, an enzyme involved in lipid metabolism and implicated in various diseases, including cancer and neurodegenerative disorders. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to ACSL4, this antibody enables accurate detection and analysis of the enzyme in different cell types, making it ideal for investigations into lipid metabolism, cancer biology, and neurological diseases.
ACSL4 plays a crucial role in lipid synthesis and oxidation, impacting cellular processes such as proliferation, apoptosis, and inflammation. Dysregulation of ACSL4 has been linked to tumorigenesis, neurodegeneration, and other pathological conditions, highlighting its potential as a therapeutic target. By studying the expression and function of ACSL4 using this antibody, researchers can gain valuable insights into disease mechanisms and potentially identify new treatment strategies.
Product Name:
ACSL4 Rabbit Polyclonal Antibody
SKU:
CAB6826
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1?).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:
ACS4; FACL4; LACS4; MRX63; MRX68; XLID63; ACSL4
Positive Sample:
HepG2,Hep G2
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein.
Calculated MW:
79kDa
Observed MW:
79kDa/74kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2182
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells using ACSL4 Rabbit pAb(CAB6826) at 1:400 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:10s.
