The ACSL1 Polyclonal Antibody (CAB16253) is a valuable tool for researchers studying the ACSL1 protein, a key enzyme involved in fatty acid metabolism. This antibody, derived from rabbits, exhibits high reactivity specifically with human samples and has been validated for use in Western blot applications. By targeting the ACSL1 protein, this antibody allows for the detection and analysis of ACSL1 expression in various cell types, making it an ideal choice for investigations in metabolism, lipid signaling, and metabolic disorders.
ACSL1, also known as Acyl-CoA Synthetase Long Chain Family Member 1, plays a crucial role in fatty acid activation and lipid biosynthesis processes within cells. Dysregulation of ACSL1 has been linked to metabolic diseases such as obesity, diabetes, and cardiovascular disorders, making it a promising target for therapeutic interventions. By elucidating the functions of ACSL1, researchers can gain insights into metabolic pathways and potentially identify novel treatment strategies for metabolic disorders.
Product Name:
ACSL1 Rabbit Polyclonal Antibody
SKU:
CAB16253
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 46-145 of human ACSL1 (NP_001986.2).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ACS1; LACS; FACL1; FACL2; LACS1; LACS2; ACSL1
Positive Sample:
Raji,Mouse kidney,Rat heart
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein.
Calculated MW:
78kDa
Observed MW:
78kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2180
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACSL1 Rabbit pAb (CAB16253) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
