The ACPP Polyclonal Antibody (CAB1937) is a crucial tool for researchers studying Acid Phosphatase, Prostate. This antibody, produced in rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry. It specifically targets the ACPP protein, allowing for accurate detection and analysis in a variety of biological samples.ACPP, an enzyme expressed in the prostate gland, has been identified as a biomarker for prostate cancer and other prostate-related diseases.
The antibody's high reactivity with human samples makes it an essential asset for studies in cancer research and diagnostics. By understanding the expression and activity of ACPP, researchers can gain insights into the progression and pathology of prostate diseases, potentially leading to improved diagnostic tools and therapeutic strategies.
Cell membrane, Lysosome membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
45kDa
Observed MW:
50kDa
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway.
Purification Method:
Affinity purification
Gene ID:
55
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (CAB1937) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
