The ACP2 Polyclonal Antibody (PAC018511) is a valuable tool for researchers studying ACP2, a lysosomal enzyme involved in various cellular processes. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications. By binding specifically to the ACP2 protein, this antibody enables precise detection and analysis in a variety of cell types, facilitating research in fields such as biochemistry and molecular biology.
ACP2, also known as acid phosphatase 2, plays a crucial role in lysosomal function and maintenance of cellular homeostasis. Dysregulation of ACP2 activity has been implicated in diseases such as lysosomal storage disorders, making it a potential therapeutic target for drug development. Understanding the function and regulation of ACP2 is essential for unraveling its role in disease pathogenesis and developing targeted interventions to treat associated disorders.
Antibody Name:
ACP2 Antibody (PACO18511)
Antibody SKU:
PACO18511
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human ACP2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: A431 cells, A549 cells, hela cells, Primary antibody: PACO18511(ACP2 Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds.
Background:
This gene encodes the β subunit of lysosomal acid, phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid, phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related α subunit gene cause acid, phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Synonyms:
acid, phosphatase 2, lysosomal
UniProt Protein Function:
ACP2: Defects in ACP2 are a cause of acid phosphatase deficiency (ACPHD). The clinical features are intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding, and death in early infancy. Lysosomal acid phosphatase is deficient in cultured fibroblasts and multiple tissues. Belongs to the histidine acid phosphatase family.Protein type: Membrane protein, integral; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 11p11.2|11p12-p11Cellular Component: lysosomal lumen; membrane; lysosome; lysosomal membrane; integral to membraneMolecular Function: acid phosphatase activityBiological Process: dephosphorylation; lysosome organization and biogenesis; skeletal developmentDisease: Acid Phosphatase Deficiency
UniProt Protein Details:
NCBI Summary:
This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. LAP-deficiencies in mice cause multiple defects including bone structure alterations, lysosomal storage defects in the kidneys and central nervous system, and an increased tendency towards seizures. An enzymatically-inactive allele of LAP in mice exhibited a more severe phenotype than the null allele, and defects included cerebellum abnormalities, growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
