Anti-ACOX1 Antibody (CAB8091)

Antibody Name:	ACOX1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8091
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACOX1 (NP_004026.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	NCI-H460, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACOX1 (NP_004026.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNPD LRRE RDSA SFNP ELLT HILD GSPE KTRR RREI ENMI LNDP DFQH EDLN FLTR SQRY EVAV RKSA IMVK KMRE FGIA DPDE IMWF KNFV HRGR PEPL DLHL GMFL PTLL HQAT AEQQ ERFF MPAW NLEI IGTY AQTE MGHG THLR GLET TATY DPET QEFI LNSP TVTS IKWW PGGL GKTS NHAI VLAQ LITK GKCY GLHA FIVP IREI GTHK PLPG ITVG DIGP KFGY DEID NGYL KMDN HRIP RENM LMKY AQVK PDGT YVKP LS
Gene ID:	51
Uniprot:	Q15067
Cellular Location:	Peroxisome
Calculated MW:	70kDa/74kDa
Observed MW:	74kDa

Synonyms:

ACOX1, ACOX, PALMCOX, SCOX

Background:

The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	ACOX1: Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA. Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD); also known as peroxisomal acyl-CoA oxidase deficiency. Pseudo-NALD is a peroxisomal single-enzyme disorder. Clinical features include mental retardation, leukodystrophy, seizures, mild hepatomegaly, hearing deficit. Pseudo-NALD is characterized by increased plasma levels of very-long chain fatty cids, due to decreased or absent peroxisome acyl-CoA oxidase activity. Peroxisomes are intact and functioning. Belongs to the acyl-CoA oxidase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.3.3.6; Oxidoreductase; Lipid Metabolism - unsaturated fatty acid biosynthesis; Lipid Metabolism - fatty acid; Lipid Metabolism - alpha-linolenic acid Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: intracellular membrane-bound organelle; membrane; nucleolus; nucleoplasm; nucleus; peroxisomal matrix; peroxisome; plasma membrane Molecular Function:acyl-CoA binding; acyl-CoA dehydrogenase activity; acyl-CoA oxidase activity; electron carrier activity; PDZ domain binding; protein N-terminus binding; receptor binding Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase; fatty acid beta-oxidation using acyl-CoA oxidase; fatty acid oxidation; generation of precursor metabolites and energy; lipid metabolic process; prostaglandin metabolic process; very-long-chain fatty acid metabolic process Disease: Peroxisomal Acyl-coa Oxidase Deficiency
NCBI Summary:	The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15067
NCBI GenInfo Identifier:	126302511
NCBI Gene ID:	51
NCBI Accession:	Q15067.3
UniProt Secondary Accession:	Q15067,Q12863, Q15068, Q15101, Q16131, Q7Z3W5, Q9UD31 A8K6X8, A8KAA0, B4DK61, F5GYQ8,
UniProt Related Accession:	Q15067
Molecular Weight:	70,136 Da
NCBI Full Name:	Peroxisomal acyl-coenzyme A oxidase 1
NCBI Synonym Full Names:	acyl-CoA oxidase 1
NCBI Official Symbol:	ACOX1
NCBI Official Synonym Symbols:	ACOX; SCOX; PALMCOX
NCBI Protein Information:	peroxisomal acyl-coenzyme A oxidase 1
UniProt Protein Name:	Peroxisomal acyl-coenzyme A oxidase 1
UniProt Synonym Protein Names:	Palmitoyl-CoA oxidase; Straight-chain acyl-CoA oxidase; SCOX
Protein Family:	Peroxisomal acyl-coenzyme A oxidase
UniProt Gene Name:	ACOX1
UniProt Entry Name:	ACOX1_HUMAN

	Western blot analysis of extracts of various cell lines, using ACOX1 antibody (CAB8091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse kidney using ACOX1 Rabbit pAb (CAB8091) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using ACOX1 antibody (CAB8091) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)