The ACOT8 Polyclonal Antibody (CAB13067) is a high-quality research tool designed for studying ACOT8, a key enzyme involved in fatty acid metabolism. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ACOT8 protein, researchers can reliably detect and analyze its levels in a variety of cell types, making it ideal for investigations in metabolic disorders, lipid metabolism, and obesity-related research.
ACOT8, also known as acyl-CoA thioesterase 8, plays a crucial role in lipid catabolism by catalyzing the hydrolysis of acyl-CoA thioesters. Its involvement in fatty acid metabolism makes it an important target for studying processes such as beta-oxidation and energy production. Research on ACOT8 can provide insights into metabolic disorders, obesity, and related conditions, ultimately contributing to the development of new therapeutic strategies for managing these diseases.
Product Name:
ACOT8 Rabbit Polyclonal Antibody
SKU:
CAB13067
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACOT8 (NP_005460.2).
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells.
Purification Method:
Affinity purification
Gene ID:
10005
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACOT8 Rabbit pAb (CAB13067) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
