Anti-ACOT1 Antibody (PACO56406)

Antibody Name:	ACOT1 Antibody (PACO56406)
Antibody SKU:	PACO56406
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Acyl-coenzyme A thioesterase 1 protein (258-350AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO56406 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with PACO56406 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid, and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acid, and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity).
Synonyms:	Acyl-coenzyme A thioesterase 1 (Acyl-CoA thioesterase 1) (EC 3.1.2.2) (CTE-I) (CTE-Ib) (Inducible cytosolic acyl-coenzyme A thioester hydrolase) (Long chain acyl-CoA thioester hydrolase) (Long chain acyl-CoA hydrolase), ACOT1, CTE1

UniProt Protein Function:	ACOT1: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16. Belongs to the C/M/P thioester hydrolase family.Protein type: EC 3.1.2.2; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesisChromosomal Location of Human Ortholog: 14q24.3Cellular Component: cytosolMolecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activityBiological Process: acyl-CoA metabolic process; very-long-chain fatty acid metabolic process; long-chain fatty acid metabolic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q86TX2
NCBI GenInfo Identifier:	81230485
NCBI Gene ID:	641371
NCBI Accession:	NP_001032238.1
UniProt Secondary Accession:	Q86TX2,Q3I5F9, A1L173
UniProt Related Accession:	Q86TX2
Molecular Weight:	46,277 Da
NCBI Full Name:	acyl-coenzyme A thioesterase 1
NCBI Synonym Full Names:	acyl-CoA thioesterase 1
NCBI Official Symbol:	ACOT1
NCBI Official Synonym Symbols:	ACH2; CTE-1; LACH2
NCBI Protein Information:	acyl-coenzyme A thioesterase 1; CTE-I; CTE-Ib; long chain acyl-CoA hydrolase; long chain acyl-CoA thioester hydrolase; inducible cytosolic acyl-coenzyme A thioester hydrolase
UniProt Protein Name:	Acyl-coenzyme A thioesterase 1
UniProt Synonym Protein Names:	CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase
Protein Family:	Acyl-coenzyme A thioesterase
UniProt Gene Name:	ACOT1
UniProt Entry Name:	ACOT1_HUMAN

Antibodies

ACOT1 Antibody (PACO05017)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)