The ACOT1 Polyclonal Antibody (PAC023373) is a valuable tool for researchers studying ACOT1, an enzyme involved in lipid metabolism and energy production. This antibody is produced in rabbits and is highly specific for ACOT1 in human samples, making it a reliable option for Western blot applications. By binding to the ACOT1 protein, this antibody enables researchers to detect and analyze ACOT1 expression levels in various cell types, making it ideal for studies in metabolism, lipid signaling, and energy regulation.ACOT1, also known as Acyl-CoA Thioesterase 1, plays a crucial role in lipid metabolism by hydrolyzing acyl-CoA to free fatty acids and CoA, a process essential for energy production in cells. Dysregulation of ACOT1 has been linked to metabolic disorders, fatty liver disease, and obesity, highlighting its importance in maintaining metabolic homeostasis.
Investigating the function and regulation of ACOT1 is critical for understanding its role in metabolic diseases and developing targeted therapies to modulate its activity for potential therapeutic interventions.In conclusion, the ACOT1 Polyclonal Antibody is a valuable tool for researchers interested in studying lipid metabolism, energy regulation, and metabolic diseases. Its high specificity and reliability make it a trusted choice for Western blot applications, providing essential insights into the role of ACOT1 in cellular metabolism and disease pathogenesis.
Synthesized peptide derived from internal of human ACOT1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, using ACOT1 antibody.
Immunofluorescence analysis of MCF-7 cells, using ACOT1 antibody.
Background:
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 By similarity.
Synonyms:
ACH2; CTE-1; EC 3.1.2.2; LACH2;
UniProt Protein Function:
ACOT1: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16. Belongs to the C/M/P thioester hydrolase family.Protein type: EC 3.1.2.2; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesisChromosomal Location of Human Ortholog: 14q24.3Cellular Component: cytosolMolecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activityBiological Process: acyl-CoA metabolic process; very-long-chain fatty acid metabolic process; long-chain fatty acid metabolic process
