The ACOT1 Polyclonal Antibody (PAC015260) is a valuable tool for researchers studying ACOT1, an important enzyme involved in lipid metabolism. This antibody, raised in rabbits, has been shown to be highly specific for detecting ACOT1 in human samples and is validated for use in Western blot applications. By binding to the ACOT1 protein, this antibody allows for the precise detection and analysis of ACOT1 in various cell types, making it ideal for studies in biochemistry and metabolic research.ACOT1, also known as acyl-CoA thioesterase 1, plays a critical role in lipid metabolism by catalyzing the hydrolysis of acyl-CoA esters to free fatty acids and coenzyme A.
Dysregulation of ACOT1 has been implicated in various metabolic disorders, making it a promising target for therapeutic interventions. Understanding the function of ACOT1 is crucial for developing treatments for conditions such as obesity, diabetes, and cardiovascular disease.Overall, the ACOT1 Polyclonal Antibody is a valuable tool for researchers working in the fields of biochemistry, metabolism, and drug development. Its high specificity and sensitivity make it an ideal choice for studying the role of ACOT1 in various physiological and pathological processes.
Antibody Name:
ACOT1 Antibody (PACO15260)
Antibody SKU:
PACO15260
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Fusion protein of human ACOT1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO15260(ACOT1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Acyl-CoA thioesterases such as ACOT1, hydrolyze acyl-CoAs to the free fatty acid, and CoA. ACOTs therefore play key roles in maintaining the intracellular ratio between CoA esters of various lipids and free fatty acid, Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid, and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acid, and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.
Synonyms:
acyl-CoA thioesterase 1
UniProt Protein Function:
ACOT1: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16. Belongs to the C/M/P thioester hydrolase family.Protein type: EC 3.1.2.2; Hydrolase; Lipid Metabolism - unsaturated fatty acid biosynthesisChromosomal Location of Human Ortholog: 14q24.3Cellular Component: cytosolMolecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activityBiological Process: acyl-CoA metabolic process; very-long-chain fatty acid metabolic process; long-chain fatty acid metabolic process
