The ACO2 Polyclonal Antibody (CAB3716) is specifically designed for research involving the ACO2 protein, an essential enzyme in the citric acid cycle involved in cellular energy production. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ACO2 protein, this antibody enables the detection and analysis of ACO2 in various cell types, making it an ideal tool for studies related to metabolism, bioenergetics, and mitochondrial function.
ACO2 is a key enzyme in the citric acid cycle, also known as the Krebs cycle, which generates ATP, the energy currency of the cell. Dysregulation of ACO2 has been associated with metabolic disorders, neurodegenerative diseases, and cancer, making it a crucial target for research aimed at understanding and potentially treating these conditions. By studying the role of ACO2 in cellular metabolism, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.
Product Name:
Aconitase 2 (ACO2) Rabbit Polyclonal Antibody
SKU:
CAB3716
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human Aconitase 2 (Aconitase 2 (ACO2)) (NP_001089.1).
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
Purification Method:
Affinity purification
Gene ID:
50
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Aconitase 2 (ACO2) Rabbit pAb (CAB3716) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
