The ACO1 Polyclonal Antibody (CAB7867) is a valuable tool for researchers studying ACO1, a key enzyme involved in iron metabolism and cellular respiration. This antibody, produced in rabbits, is highly specific and compatible with human samples for use in Western blot applications. By binding to the ACO1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in biochemistry, metabolism, and diseases related to iron dysregulation.
ACO1, also known as aconitase 1, is essential for the maintenance of cellular iron homeostasis and plays a crucial role in the Krebs cycle, a vital process in energy production. Dysregulation of ACO1 has been implicated in various diseases such as iron overload disorders, neurodegenerative diseases, and cancer. Investigating the function and regulation of ACO1 is essential for understanding the underlying mechanisms of these conditions and developing targeted therapies.
Product Name:
IREB1 Rabbit Polyclonal Antibody
SKU:
CAB7867
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human Aconitase 1 (ACO1) (NP_002188.1).
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
Purification Method:
Affinity purification
Gene ID:
48
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Aconitase 1 (ACO1) antibody (CAB7867) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
