Anti-IREB1 Antibody (CAB6008)

Antibody Name:	Anti-IREB1 Antibody
Antibody SKU:	CAB6008
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human IREB1

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney, Mouse ovary, Rat kidney

Immunogen:	A synthetic peptide of human IREB1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	48
Uniprot:	P21399
Cellular Location:	Cytoplasm
Calculated MW:	98kDa
Observed MW:	100kDa

Synonyms:

ACO1, ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1

Background:

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants

UniProt Protein Function:	IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.
UniProt Protein Details:	Protein type:EC 4.2.1.3; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; RNA-binding; Lyase; Endoplasmic reticulum; Translation Chromosomal Location of Human Ortholog: 9p21.1 Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion Molecular Function:4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; protein binding; RNA binding Biological Process: citrate metabolic process; response to iron(II) ion
NCBI Summary:	The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
UniProt Code:	P21399
NCBI GenInfo Identifier:	3123225
NCBI Gene ID:	48
NCBI Accession:	P21399.3
UniProt Secondary Accession:	P21399,Q14652, Q5VZA7, D3DRK7,
UniProt Related Accession:	P21399
Molecular Weight:	98,399 Da
NCBI Full Name:	Cytoplasmic aconitate hydratase
NCBI Synonym Full Names:	aconitase 1
NCBI Official Symbol:	ACO1
NCBI Official Synonym Symbols:	IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
NCBI Protein Information:	cytoplasmic aconitate hydratase
UniProt Protein Name:	Cytoplasmic aconitate hydratase
UniProt Synonym Protein Names:	Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Protein Family:	Aconitate hydratase
UniProt Gene Name:	ACO1
UniProt Entry Name:	ACOC_HUMAN

	Western blot analysis of extracts of various cell lines, using IREB1 antibody (CAB6008) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using IREB1 antibody (CAB6008) (40x lens).
	Immunohistochemistry of paraffin-embedded rat lung using IREB1 antibody (CAB6008) (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using IREB1 antibody (CAB6008) (40x lens).

Antibodies	ELISA Kits
Anti-IREB1 Antibody (CAB7867)	Human Aconitase 1 ELISA Kit
Phospho-ACO1 (S711) Antibody (PACO02931)	Human ACO1 (Aconitase 1) CLIA Kit (HUES00249)
Phospho-ACO1 (S138) Antibody (PACO04442)	Human ACO1 (Aconitase 1) ELISA Kit (HUES01568)
ACO1 Antibody (PACO04443)
ACO1 Antibody (PACO04444)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)