The Acid Ceramidase Polyclonal Antibody (CAB6527) is a valuable tool for researchers studying acid ceramidase, an enzyme involved in sphingolipid metabolism and cell signaling pathways. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications.Acid ceramidase plays a crucial role in the breakdown of ceramides, which are lipid molecules involved in various cellular processes such as apoptosis, inflammation, and cell proliferation. Dysregulation of acid ceramidase has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.
Therefore, studying the expression and activity of acid ceramidase is essential for understanding the role of sphingolipids in health and disease.Researchers can confidently use the Acid Ceramidase Polyclonal Antibody to detect and analyze acid ceramidase levels in different cell types and tissues. Its high specificity and sensitivity make it an ideal tool for investigating the function of acid ceramidase in various biological contexts, ultimately leading to a better understanding of sphingolipid metabolism and its implications in disease pathology.
Product Name:
ASAH1 Rabbit Polyclonal Antibody
SKU:
CAB6527
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 140-389 of human ASAH1 (NP_001120977.1).
This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy.
Purification Method:
Affinity purification
Gene ID:
427
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ASAH1 antibody (CAB6527) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
