The Acetyl-NFE4-K43 Polyclonal Antibody (PACO06074) is a valuable tool for researchers studying post-translational modifications of the NFE4 protein, specifically acetylation at lysine 43. This antibody, produced in rabbits, has high specificity and sensitivity for detecting acetylated NFE4 in human samples, making it suitable for Western blot applications.NFE4 is a transcription factor that plays a crucial role in regulating gene expression in response to various stimuli. Acetylation of NFE4 at lysine 43 is known to impact its activity, potentially influencing downstream gene regulation processes. By using the Acetyl-NFE4-K43 Polyclonal Antibody, researchers can investigate the role of this specific modification in cellular signaling pathways and disease mechanisms.
Research areas that may benefit from the use of this antibody include epigenetics, cancer biology, and cell signaling. Understanding the impact of acetylation on NFE4 function could provide insights into diseases where dysregulation of gene expression is a contributing factor. By studying acetylated NFE4, researchers may uncover new therapeutic targets for conditions such as cancer, neurodegenerative disorders, and inflammatory diseases.
Antibody Name:
Acetyl-NFE4 (K43) Antibody (PACO06074)
Antibody SKU:
PACO06074
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:20000, WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from the human NF-E4 around the acetylation site of K43.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody.
Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody.
Synonyms:
NFE4; Transcription factor NF-E4
UniProt Protein Function:
NF-E4: Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma- globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter. 2 isoforms of the human protein are produced by alternative initiation.Chromosomal Location of Human Ortholog: 7q22.1
UniProt Protein Details:
NCBI Summary:
The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008]
