The Acetyl-Histone H4 (K91) Polyclonal Antibody (CAB7409) is a valuable tool for researchers studying epigenetics and chromatin modifications. This antibody, raised in rabbits, specifically targets the acetylation of histone H4 at lysine 91, a critical post-translational modification involved in gene regulation and chromatin remodeling.Histone acetylation at lysine 91 of histone H4 has been linked to various biological processes, including DNA repair, transcriptional activation, and cell cycle regulation. The CAB7409 antibody has been validated for use in Western blot applications, enabling detection and analysis of acetylated histone H4 (K91) in various cell types and tissue samples.
Understanding the role of acetyl-histone H4 (K91) in gene expression and chromatin dynamics is essential for unraveling the complexities of epigenetic regulation and its implications in diseases such as cancer, neurological disorders, and developmental disorders. The CAB7409 antibody provides researchers with a reliable tool for investigating the impact of histone acetylation at lysine 91 on gene expression and chromatin structure, opening new possibilities for therapeutic interventions targeting epigenetic pathways.
Product Name:
Acetyl-Histone H4-K91 Rabbit Polyclonal Antibody
SKU:
CAB7409
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K91 of human Histone H4 (NP_003529.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K91 Rabbit pAb (CAB7409) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 300s.
