The Acetyl-Histone H4 (K8) Polyclonal Antibody (CAB7258) is a valuable tool for researchers studying histone modifications and epigenetic regulation. This antibody, raised in rabbits, has high reactivity with acetylated histone H4 (lysine 8) in human samples, making it ideal for use in Western blot applications. By specifically binding to acetyl-histone H4 (K8), this antibody allows for the detection and analysis of histone modifications in various cell types, enabling further exploration into the role of epigenetic regulation in gene expression and cellular function.Histone modifications, such as acetylation, play a crucial role in gene regulation by altering chromatin structure and accessibility to transcription factors.
Acetylation of histone H4 at lysine 8 is known to be involved in regulating gene expression and is implicated in various cellular processes, including DNA repair, cell cycle progression, and apoptosis. Understanding the impact of acetyl-histone H4 (K8) on gene regulation can provide insights into developmental processes, disease mechanisms, and potential therapeutic targets in cancer and other pathologies.Overall, the Acetyl-Histone H4 (K8) Polyclonal Antibody (CAB7258) is a reliable tool for researchers interested in exploring the role of histone modifications in epigenetic regulation and gene expression. Its high reactivity and specificity make it a valuable asset for studies in chrom
Product Name:
Acetyl-Histone H4-K8 Rabbit Polyclonal Antibody
SKU:
CAB7258
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K8 of human Histone H4 (NP_003529.1).
Sequence:
GGKG L
Tested Applications:
WBIHC-PIF/ICCChIPChIP-seqELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:500 - 1:1000 ChIP,5μg antibody for 5μg-10μg of ChromatinChIP-seq,1:20 - 1:50
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H4-K8 Rabbit pAb (CAB7258) at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
