The Acetyl-Histone H4 (K5) Rabbit Monoclonal Antibody (CAB19525) is a highly specific antibody designed for research involving acetylated histone H4 at lysine 5, an important post-translational modification associated with gene regulation and chromatin remodeling. The antibody, raised in rabbits, is validated for use in various applications, including immunofluorescence and chromatin immunoprecipitation (ChIP) assays. It specifically recognizes acetylated histone H4 at lysine 5, allowing for accurate detection and quantification in experimental samples.Acetylation of histone H4 at lysine 5 is known to play a role in gene transcription and is associated with active chromatin states. Studies have shown that dysregulation of histone acetylation patterns, including acetylation at lysine 5, is linked to various diseases, including cancer and neurological disorders.
Understanding the function and regulation of acetylated histone H4 at lysine 5 is important for unraveling the molecular mechanisms underlying these diseases and developing potential therapeutic strategies.The Acetyl-Histone H4 (K5) Rabbit Monoclonal Antibody (CAB19525) provides researchers with a reliable tool to study the role of histone acetylation in gene expression and chromatin structure. Its high specificity and sensitivity make it an essential reagent for investigations into epigenetic mechanisms and their implications in health
Product Name:
Acetyl-Histone H4-K5 Rabbit Monoclonal Antibody
SKU:
CAB19525
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K5 of human Histone H4 (P62805).
HeLa treated by TSA, NIH/3T3 treated by TSA, C6 treated by TSA
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, nucleus
Calculated MW:
11kDa
Observed MW:
11kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Purification Method:
Affinity purification
Gene ID:
8359
Clone Number:
ARC0002
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H4-K5 Rabbit mAb (CAB19525) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
