The Acetyl-Histone H4 (K5) Polyclonal Antibody (CAB15233) is essential for research involving epigenetics and chromatin modifications. This antibody is raised in rabbits and specifically targets the acetylation of histone H4 lysine 5, a key regulatory event in gene expression. Highly reactive with human samples, this antibody is validated for use in Western blot applications, allowing for precise detection and analysis of acetylated histone H4 (K5) levels in various cell types. Its specificity for this modification makes it an ideal tool for studying the epigenetic regulation of gene expression in the fields of cancer research, epigenetics, and developmental biology.
The modification of histones plays a crucial role in controlling gene expression patterns and has been implicated in various diseases including cancer. Understanding the role of acetylation at specific histone residues like histone H4 lysine 5 is essential for unraveling the complex mechanisms of gene regulation and identifying potential therapeutic targets. The Acetyl-Histone H4 (K5) Polyclonal Antibody is a valuable tool for researchers investigating the epigenetic basis of diseases and developing novel treatments targeting chromatin modifications.
Product Name:
Acetyl-Histone H4-K5 Rabbit Polyclonal Antibody
SKU:
CAB15233
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_001029249.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H4-K5 Rabbit pAb (CAB15233) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
