The Acetyl-Histone H4 (K16) Polyclonal Antibody (CAB5280) is specifically designed for researchers studying histone modifications and chromatin structure. This antibody, produced in rabbits, is highly reactive with acetylated histone H4 at lysine 16 in human samples, making it a valuable tool for epigenetics and gene regulation studies.Histone acetylation at lysine 16 of histone H4 is known to be involved in gene activation and chromatin remodeling. This modification plays a crucial role in regulating gene expression and can impact various cellular processes, including proliferation, differentiation, and DNA repair.
The Acetyl-Histone H4 (K16) antibody allows for the detection and analysis of this important histone modification in a variety of cell types, providing insights into the epigenetic mechanisms underlying diseases such as cancer and neurological disorders.By enabling researchers to study the dynamics of histone acetylation at lysine 16 of histone H4, this antibody contributes to our understanding of epigenetic regulation and its impact on gene function. Its specificity and reliability make it an essential tool for investigations into the role of histone modifications in health and disease.
Product Name:
Acetyl-Histone H4-K16 Rabbit Polyclonal Antibody
SKU:
CAB5280
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Acetyl-Histone H4-K16 Rabbit pAb (CAB5280) at 1:400 dilution.NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
