The Acetyl-Histone H4 (K12) Polyclonal Antibody (CAB14227) is a valuable tool for researchers studying epigenetics and histone modifications. This antibody, raised in rabbits, specifically targets the acetyl-histone H4 at lysine 12, allowing for the detection and analysis of this important histone modification in human samples. Validated for use in Western blot applications, the CAB14227 antibody enables researchers to investigate the role of acetyl-histone H4 (K12) in gene expression and chromatin structure in various cell types.Histone modifications, such as acetylation, play a crucial role in regulating gene expression and chromatin remodeling. Acetyl-histone H4 at lysine 12 is known to be involved in transcriptional activation and is associated with active gene promoters.
Understanding the dynamics of this histone modification can provide insights into cellular processes such as DNA repair, cell differentiation, and development.The CAB14227 antibody is a powerful tool for researchers interested in unraveling the complex mechanisms of epigenetic regulation and its impact on various biological processes. By targeting acetyl-histone H4 at lysine 12, this antibody opens up new possibilities for studying the role of histone modifications in health and disease, making it an essential reagent for studies in epigenetics, cancer research, and developmental biology.
Product Name:
Acetyl-Histone H4-K12 Rabbit Polyclonal Antibody
SKU:
CAB14227
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H4-K12 Rabbit pAb (CAB14227) at 1:500 dilution.HeLa cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
