The Acetyl-Histone H3 (K9) Polyclonal Antibody (CAB7255) is a valuable tool for researchers studying modifications of histone proteins in epigenetics. Histone H3 lysine 9 acetylation plays a crucial role in gene activation and chromatin remodeling, making this antibody essential for studying gene expression regulation.Raised in rabbits, this antibody is highly specific to acetyl-histone H3 (K9) and is validated for use in various applications, such as Western blotting and chromatin immunoprecipitation (ChIP). It allows for precise detection and analysis of acetyl-histone H3 (K9) levels, providing valuable insights into epigenetic mechanisms in different cell types and experimental conditions.
Acetyl-histone H3 (K9) has been implicated in a wide range of biological processes, including cell differentiation, development, and disease progression. Therefore, this antibody is a powerful tool for researchers investigating epigenetic regulation in cancer, stem cell biology, and other fields where chromatin modifications play a key role.By enabling researchers to study the dynamics of acetyl-histone H3 (K9) in a specific and sensitive manner, this antibody contributes to advancing our understanding of epigenetic mechanisms and their impact on cellular function and disease development. Discover the potential of epigenetics with the Acetyl-Histone H3 (K9) Polyclonal Antibody (CAB725
Product Name:
Acetyl-Histone H3-K9 Rabbit Polyclonal Antibody
SKU:
CAB7255
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H3 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9 Rabbit pAb (CAB7255) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
