The Acetyl-Histone H3 (K9/K14/K18/K23/K27) Rabbit Polyclonal Antibody (CAB17917) is a valuable tool for researchers studying epigenetics and chromatin remodeling. This antibody, raised in rabbits, specifically targets acetylated forms of Histone H3 at multiple lysine residues, allowing for the detection and analysis of histone modifications involved in gene expression regulation.Histone H3 acetylation at these sites is known to be associated with active transcription and chromatin accessibility, making this antibody an essential reagent for studies in gene regulation, cell differentiation, and development. Validated for use in applications such as ChIP, Western blot, and immunofluorescence, this antibody is highly reactive with human samples, providing reliable and consistent results for researchers working in the field of molecular biology.
By targeting multiple acetylated lysine residues on Histone H3, the CAB17917 antibody enables researchers to investigate the dynamic interplay between chromatin modifications and gene expression, offering insights into the mechanisms underlying various biological processes. Its versatility and specificity make it an indispensable tool for unraveling the complexities of epigenetic regulation in health and disease.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb (CAB17917) at 1:5000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
