The Acetyl-Histone H3 (K56) Polyclonal Antibody (CAB7256) is a valuable tool for researchers studying epigenetics and chromatin modifications. This antibody, raised in rabbits, is highly specific and reactive with acetylated Histone H3 at lysine 56 in human samples. Validated for use in Western blot applications, this antibody allows for precise detection and analysis of acetylation at this specific site.Histone acetylation at lysine 56 has been linked to various cellular processes, including DNA repair, transcriptional regulation, and cell cycle progression. Dysregulation of this modification has been associated with various diseases, including cancer and neurological disorders.
By targeting acetylated Histone H3 at lysine 56, researchers can gain insights into the role of epigenetic modifications in disease development and progression.Overall, the Acetyl-Histone H3 (K56) Polyclonal Antibody (CAB7256) is a valuable tool for studying histone acetylation dynamics and its impact on cellular function. Its specificity and sensitivity make it an ideal choice for researchers investigating epigenetic mechanisms in health and disease.
Product Name:
Acetyl-Histone H3-K56 Rabbit Polyclonal Antibody
SKU:
CAB7256
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K56 of human Histone H3 (NP_003520.1).
Sequence:
YQKS T
Tested Applications:
WBIF/ICCChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K56 Rabbit pAb (CAB7256) at 1:10000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
