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Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Polyclonal Antibody (CAB21295)

SKU:
CAB21295
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $587
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Polyclonal Antibody
SKU:CAB21295
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic acetylated peptide around K4 & K27 of human Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 (NP_003520.1).
Sequence:TKQT ARKS TGGK APRK QLAT KAAR
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Acetyl-Histone H3-K4/K9/K14/K18/K23/K27
Positive Sample:HeLa treated by TSA,NIH/3T3 treated by TSA,C6 treated by TSA,H3 protein
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:8290/8350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Rabbit pAb (CAB21295) at 1:600 dilution.HeLa cells,NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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