The Acetyl-Histone H3 (K23) Rabbit Polyclonal Antibody (CAB18154) is a powerful tool for researchers studying epigenetic modifications involved in gene regulation. This antibody, raised in rabbits, is highly specific to acetylated histone H3 at lysine 23 and is validated for use in various applications, including Western blotting and chromatin immunoprecipitation (ChIP).Histone acetylation at lysine 23 of histone H3 is known to play a role in gene activation and regulation. By targeting this specific acetylation site, researchers can gain insights into the mechanisms underlying gene expression, cell differentiation, and other biological processes.
This antibody allows for the detection and analysis of acetyl-histone H3 (K23) in various cell types and tissue samples, making it an invaluable tool for epigenetics research. By understanding the role of histone acetylation in gene regulation, researchers can uncover potential therapeutic targets for diseases such as cancer, neurological disorders, and metabolic conditions.
Product Name:
Acetyl-Histone H3-K23 Rabbit Polyclonal Antibody
SKU:
CAB18154
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K23 of human Histone H3 (NP_003520.1).
Sequence:
ATKA A
Tested Applications:
WBIHC-PIF/ICCChIPELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit pAb (CAB18154) at 1:500 dilution.HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
