The Acetyl-Histone H3 (K18) Polyclonal Antibody (CAB7257) offered by AssayGenie is a high-quality tool for researchers studying epigenetic modifications involved in gene regulation. This antibody, raised in rabbits, specifically targets acetylation at lysine 18 of the histone H3 protein, a modification associated with active gene transcription.Validated for Western blot applications and highly reactive with human samples, this antibody enables accurate detection and analysis of acetylated histone H3 (K18) in a variety of cell types. Its specificity for this modification makes it a valuable tool for investigating the role of histone acetylation in gene expression, chromatin remodeling, and various cellular processes.
Histone acetylation at lysine 18 of histone H3 is known to be involved in the regulation of gene expression, cell differentiation, and development. Dysregulation of this epigenetic modification has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. By targeting acetylated histone H3 (K18), researchers can gain insights into the mechanisms underlying these diseases and potentially identify targets for therapeutic interventions.Overall, the Acetyl-Histone H3 (K18) Polyclonal Antibody (CAB7257) is an essential tool for epigenetics research, offering reliable and specific detection of acetylated histone H3 (K18) and facilitating
Product Name:
Acetyl-Histone H3-K18 Rabbit Polyclonal Antibody
SKU:
CAB7257
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K18 of human H3 (NP_003520.1).
Sequence:
PRKQ L
Tested Applications:
WBIHC-PIF/ICCChIPELISADB
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 5μg-10μg of ChromatinDB, 1:500 - 1:1000
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (CAB7257) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
