The Acetyl-Histone H2B (Lys15) Antibody (PACO00160) is a crucial tool for researchers studying histone modifications and their impact on gene expression and chromatin structure. This rabbit polyclonal antibody is highly specific and sensitive for detecting acetylation of Histone H2B at Lysine 15 in human samples, making it an essential reagent for studies in epigenetics and cancer research.Histone acetylation at specific lysine residues is a key mechanism for regulating gene transcription and chromatin accessibility. Acetylation of Histone H2B at Lysine 15 has been linked to gene activation and is often dysregulated in cancer cells, making it a promising target for therapeutic intervention.
By targeting this specific histone modification, researchers can gain valuable insights into the epigenetic mechanisms underlying disease development and progression.The Acetyl-Histone H2B (Lys15) Antibody is validated for use in Western blot applications, enabling researchers to visualize and quantify changes in Histone H2B acetylation levels in different cell types and experimental conditions. Its high reactivity and specificity make it a reliable tool for investigating the role of histone modifications in gene regulation and disease pathogenesis.
Antibody Name:
Acetyl-Histone H2B (Lys15) Antibody
Antibody SKU:
PACO00160
Size:
50ug
Host Species:
Rabbit
Tested Applications:
WB
Recommended Dilutions:
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthetic Peptide
Form:
Liquid
Storage Buffer:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
H2B1A: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Transcribed exclusively in testis, and the corresponding protein is also present in mature sperm. Belongs to the histone H2B family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: extrinsic to plasma membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleusMolecular Function: DNA binding; protein heterodimerization activityBiological Process: establishment and/or maintenance of chromatin architecture; inflammatory response; nucleosome assembly; nucleosome disassembly; plasminogen activation; positive regulation of binding; spermatogenesis, exchange of chromosomal proteins
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
