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Acetyl-Histone H2B-K20 Polyclonal Antibody (CAB24886)

SKU:
CAB24886
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€129 - €489
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Acetyl-Histone H2B-K20 Polyclonal Antibody
SKU:CAB24886
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic acetylated peptide around K20 of human Histone H2B (NP_003519.1).
Tested Applications:WB ChIP ELISA
Recommended Dilution:WB,1:500 - 1:1000 ChIP,5μg antibody for 5μg-10μg of Chromatin
Synonyms:H2B; H2BE; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105; H2B-GL105; HIST2H2BE; Acetyl-Histone H2B-K20
Positive Sample:HeLa treated by TSA,NIH/3T3 treated by TSA,C6 treated by TSA
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:14kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.

Purification Method:Affinity purification
Gene ID:3017/8349
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit pAb (CAB24886) at 1:500 dilution. HeLa cells,NIH/3T3 cells and C6 cells were treated by TSA (1 μM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.

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