The Acetyl-Histone H2B (K15) Polyclonal Antibody (CAB15622) is a valuable tool for researchers studying histone modifications and their role in gene expression regulation. This antibody, produced in rabbits, is highly specific to the acetylated form of histone H2B at lysine 15, allowing for precise detection and analysis in various experimental systems.Histone acetylation plays a key role in chromatin remodeling and gene activation, making it crucial for understanding gene regulation processes. The acetylation of histone H2B at lysine 15 has been implicated in transcriptional activation and is associated with specific biological functions. The CAB15622 antibody binds specifically to this modification site, enabling researchers to explore the functional significance of acetyl-histone H2B (K15) in various cellular processes.
This antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, making it versatile for a wide range of research applications. By targeting acetyl-histone H2B (K15), researchers can delve deeper into the intricate mechanisms of gene regulation and epigenetic modifications, advancing our understanding of cellular processes and potentially opening doors for novel therapeutic strategies in areas such as cancer research and developmental biology.
Product Name:
Acetyl-Histone H2B-K15 Rabbit Polyclonal Antibody
SKU:
CAB15622
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K15 of human Histone H2B (NP_003519.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
3017/8349
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H2B-K15 Rabbit pAb (CAB15622) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
