The Acetyl Histone H2B (K15) Antibody (PAC002980) is a valuable tool for researchers studying epigenetics and chromatin modification. This polyclonal antibody, raised in rabbits, specifically recognizes the acetylated form of Histone H2B at lysine 15 in human samples. It has been validated for use in Western blot applications, allowing for the detection and quantification of this modified histone protein.Histone acetylation, including acetylation of Histone H2B at lysine 15, is an important epigenetic mark that regulates gene expression and chromatin structure. Aberrant acetylation patterns have been implicated in various diseases, including cancer and developmental disorders.
By using this antibody to study acetylation of Histone H2B at lysine 15, researchers can gain insights into the role of chromatin modifications in gene regulation and disease pathogenesis.This antibody is a valuable tool for scientists conducting research in the fields of epigenetics, cancer biology, and developmental biology. Its specificity for acetylated Histone H2B at lysine 15 makes it an essential reagent for exploring the dynamic and complex world of chromatin modifications.
Antibody Name:
Acetyl-Histone H2B (K15) Antibody
Antibody SKU:
PACO02980
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC, IF
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:
Human, Mouse
Immunogen:
synthesized peptide derived from human Histone H2B around the acetylation site of K15.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s
UniProt Protein Function:
H2BFS: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 21q22.3Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleusMolecular Function: DNA binding; protein heterodimerization activityBiological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly; substantia nigra development