The Histone H2B Testis-Specific Polyclonal Antibody (AB12345) is specifically designed for research involving Histone H2B, a protein involved in chromatin structure and gene regulation. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunohistochemistry. It binds specifically to the testis-specific form of Histone H2B, allowing for precise detection and analysis in tissue samples and cell lines.Histone H2B is a critical component of nucleosomes and plays a key role in regulating gene expression and DNA packaging. The testis-specific variant of Histone H2B is important for spermatogenesis and male fertility, making it an interesting target for studies in reproductive biology and developmental research.
Understanding the function and localization of this specific histone variant can provide insights into mechanisms underlying male germ cell development and fertility.Overall, the Histone H2B Testis-Specific Polyclonal Antibody is a valuable tool for researchers studying chromatin biology, gene regulation, and reproductive health. Its high specificity and sensitivity make it ideal for investigating the role of Histone H2B in various biological processes and disease conditions.
Product Name:
Histone H2B (testis specific) Polyclonal Antibody
SKU:
CAB23044
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant protein of human Histone H2B (testis specific).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Purification Method:
Affinity purification
Gene ID:
255626
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H2B (testis specific) Rabbit pAb (CAB23044) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Negative control (NC): Mouse brain,Rat brain.Exposuretime: 180s.
