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Acetyl-HIST1H2BC (K24) Antibody (PACO60549)

SKU:
PACO60549
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
ICC
IP
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Acetyl-HIST1H2BC K24 Antibody PACO60549
  • Acetyl-HIST1H2BC K24 Antibody PACO60549
  • Acetyl-HIST1H2BC K24 Antibody PACO60549
$419
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Description

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Acetyl-HIST1H2BC (K24) Antibody (PACO60549)

The Acetyl Hist1H2BC (K24) Antibody (PACO60549) is a valuable tool for research involving histone proteins, which play a crucial role in regulating gene expression and chromatin structure. This polyclonal antibody, raised in rabbits, has high reactivity with acetylated histone H2BC at lysine 24 in human samples and is validated for use in applications such as immunofluorescence and chromatin immunoprecipitation.Histone modifications, such as acetylation, are known to impact chromatin dynamics and gene transcription, making them important epigenetic markers in various biological processes.

The Acetyl Hist1H2BC (K24) Antibody specifically targets this acetylated histone variant, enabling researchers to study its role in gene regulation, DNA repair, and other cellular functions.With its ability to detect and analyze acetylated histone H2BC (K24) in different cell types, this antibody is a valuable tool for studies in epigenetics, cancer research, and developmental biology. By understanding the impact of histone modifications on gene expression, researchers can gain insights into disease mechanisms and potentially identify new targets for therapeutic intervention.

Antibody Name:Acetyl-HIST1H2BC (K24) Antibody (PACO60549)
Antibody SKU:PACO60549
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, ICC, IP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000, ICC:1:10-1:100, IP:1:200-1:2000
Species Reactivity:Human
Immunogen:Peptide sequence around site of Acetyl-Lys (24) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageImmunoprecipitating HIST1H2BC in A549 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO60549 in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO60549 (5µg) + A549 whole cell lysate (500µg). Lane 3: A549 whole cell lysate (20µg).
Product ImageImmunocytochemistry analysis of PACO60549 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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