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Acetyl-HIST1H2BC (K11) Antibody (PACO60474)

SKU:
PACO60474
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IHC
ChIP
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Acetyl-HIST1H2BC K11 Antibody PACO60474
  • Acetyl-HIST1H2BC K11 Antibody PACO60474
  • Acetyl-HIST1H2BC K11 Antibody PACO60474
€349
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Description

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Acetyl-HIST1H2BC (K11) Antibody (PACO60474)

The Acetyl-Hist1H2BC (K11) Antibody (PAC060474) is a powerful tool for researchers studying the acetylation of the histone protein Hist1H2BC at lysine 11. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting acetylated Hist1H2BC (K11) in human samples, making it a valuable asset for Western blot applications.Histone acetylation is a key epigenetic modification that plays a crucial role in gene expression regulation and chromatin structure. The acetylation of Hist1H2BC at lysine 11 has been linked to various cellular processes, including transcriptional activation and DNA repair.

By targeting this specific modification, researchers can gain insights into the mechanisms underlying these biological processes.With its ability to specifically recognize acetylated Hist1H2BC (K11), this antibody opens up new avenues for studying epigenetic regulation in a variety of contexts, ranging from cancer biology to developmental biology. Its high reactivity and validation for Western blot applications make it an indispensable tool for researchers looking to explore the role of histone acetylation in gene expression and chromatin dynamics.

Antibody Name:Acetyl-HIST1H2BC (K11) Antibody (PACO60474)
Antibody SKU:PACO60474
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, IHC, ChIP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000, IHC:1:10-1:100
Species Reactivity:Human
Immunogen:Peptide sequence around site of Acetyl-Lys (11) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Detected samples: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageChromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (PACO60474) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.
Product ImageImmunocytochemistry analysis of PACO60474 diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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