Acetyl-HIST1H1B (K16) Antibody (PACO56606)
- SKU:
- PACO56606
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IF
- ChIP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Acetyl-HIST1H1B (K16) Antibody (PACO56606)
The Acetyl-Hist1H1B (K16) Polyclonal Antibody (PAC05606) is a valuable tool for researchers studying epigenetic modifications in histones. This antibody specifically targets the acetylation of the histone 1H1B protein at lysine 16, a modification known to play a role in chromatin structure and gene regulation.Raised in rabbits, this antibody has been validated for use in various applications, including Western blotting and immunofluorescence. Its high reactivity with human samples makes it an ideal choice for researchers studying histone modifications in cancer, development, and other biological processes.
Histone acetylation is a key mechanism by which cells regulate gene expression, and aberrant acetylation patterns have been implicated in various diseases, including cancer. The Acetyl-Hist1H1B (K16) Polyclonal Antibody provides researchers with a powerful tool to investigate the role of histone acetylation in these pathological processes, ultimately contributing to our understanding of disease mechanisms and the development of potential therapeutic interventions.
| Antibody Name: | Acetyl-HIST1H1B (K16) Antibody (PACO56606) |
| Antibody SKU: | PACO56606 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF, ChIP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence around site of Acetyl-Lys (16) derived from Human Histone H1.5 |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescent analysis of HepG2 cells (sodium butyrate, 30 mM, 4h) using PACO56606 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1B (PACO56606) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter. |
| Background: | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| Synonyms: | Histone H1.5 (Histone H1a) (Histone H1b) (Histone H1s-3), HIST1H1B, H1F5 |
| UniProt Protein Function: | H1B: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: nuclear chromatin; nuclear heterochromatin; nucleosome Molecular Function:chromatin DNA binding; histone deacetylase binding Biological Process: establishment and/or maintenance of chromatin architecture; negative regulation of transcription from RNA polymerase II promoter; nucleosome assembly; positive regulation of cell growth; positive regulation of histone H3-K9 methylation; protein stabilization |
| NCBI Summary: | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P16401 |
| NCBI GenInfo Identifier: | 19856407 |
| NCBI Gene ID: | 3009 |
| NCBI Accession: | P16401.3 |
| UniProt Secondary Accession: | P16401,Q14529, Q3MJ42, |
| UniProt Related Accession: | P16401 |
| Molecular Weight: | 22,580 Da |
| NCBI Full Name: | Histone H1.5 |
| NCBI Synonym Full Names: | histone cluster 1, H1b |
| NCBI Official Symbol: | HIST1H1BÂ Â |
| NCBI Official Synonym Symbols: | H1; H1B; H1.5; H1F5; H1s-3Â Â |
| NCBI Protein Information: | histone H1.5 |
| UniProt Protein Name: | Histone H1.5 |
| UniProt Synonym Protein Names: | Histone H1a; Histone H1b; Histone H1s-3 |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H1BÂ Â |
| UniProt Entry Name: | H15_HUMAN |
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