Acetyl-CoA Colorimetric Assay Kit (MAES0238)
- SKU:
- MAES0238
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Animal Tissue
- Sensitivity:
- 150 nmol/mL
- Range:
- 150-500 nmol/mL
- Assay Time:
- 15 min
Description
system_update_altDatasheet
| Product Name: | Acetyl-CoA Colorimetric Assay Kit |
| Product Code: | MAES0238 |
| Sample Type: | Animal Tissue |
| Assay Time: | 15 min |
| Assay Type: | Quantitative |
| Synonyms: | Acetyl-CoA |
| Detection Instrument: | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 150 nmol/mL |
| Detection Range: | 150-500 nmol/mL |
| Inter-assay CV: | 0.053 |
| Intra-assay CV: | 0.04 |
Malate and NAD+ form oxoacetate and NADH under the action of malate dehydrogenase, then acetyl-coa and oxoacetate form coenzyme A and citrate under the action of citrate synthetase. The content of acetyl-coA can be calculated by measuring the change of absorbance value at 340 nm
| Storage: | -20°C |
| Valid Period: | 12 months |
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