ACER2 Antibody (PACO07237)
- SKU:
- PACO07237
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
ACER2 Antibody (PACO07237)
The ACER2 Polyclonal Antibody (PACO07237) is a valuable tool for researchers studying the ACER2 protein, which is involved in sphingolipid metabolism and regulation of cellular signaling pathways. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunofluorescence applications.ACER2, also known as alkaline ceramidase 2, plays a crucial role in regulating cellular levels of ceramides, which are important lipid molecules involved in various cellular processes such as apoptosis, proliferation, and inflammation.
Dysregulation of ceramide metabolism has been implicated in various diseases including cancer, metabolic disorders, and neurodegenerative diseases.By targeting ACER2 with this polyclonal antibody, researchers can gain insights into the function and regulation of this important enzyme, paving the way for potential therapeutic interventions targeting sphingolipid metabolism in disease states. This antibody is a valuable tool for studies in lipid biology, cell signaling, and drug discovery.
| Antibody Name: | ACER2 Antibody (PACO07237) |
| Antibody SKU: | PACO07237 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:10000, WB:1:500-1:2000, IHC-p:1:100-1:300 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Synthesized peptide derived from the Internal region of human ASAH3L. at AA rangle: 50-130 |
| Form: | Liquid |
| Storage Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200. |
 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200. |
 | Western blot analysis of COS7 using ASAH3L antibody. Secondary antibody was diluted at 1:20000. |
| Synonyms: | ACER2; ASAH3L; PP11646; Alkaline ceramidase 2; AlkCDase 2; Alkaline CDase 2; haCER2; Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like |
| UniProt Protein Function: | ACER2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. Belongs to the alkaline ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - sphingolipid; EC 3.5.1.23; HydrolaseChromosomal Location of Human Ortholog: 9p22.1Cellular Component: Golgi membrane; Golgi apparatus; integral to Golgi membraneMolecular Function: ceramidase activityBiological Process: caspase activation; response to retinoic acid; sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; negative regulation of cell-matrix adhesion; ceramide metabolic process; negative regulation of cell adhesion mediated by integrin |
| UniProt Protein Details: | |
| NCBI Summary: | The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010] |
| UniProt Code: | Q5QJU3 |
| NCBI GenInfo Identifier: | 71043498 |
| NCBI Gene ID: | 340485 |
| NCBI Accession: | NP_001010887.2 |
| UniProt Secondary Accession: | Q5QJU3,Q569G5, Q5VZR7, Q71RD2, A2A3R8 |
| UniProt Related Accession: | Q5QJU3 |
| Molecular Weight: | 15,864 Da |
| NCBI Full Name: | alkaline ceramidase 2 |
| NCBI Synonym Full Names: | alkaline ceramidase 2 |
| NCBI Official Symbol: | ACER2Â Â |
| NCBI Official Synonym Symbols: | ASAH3L; ALKCDase2Â Â |
| NCBI Protein Information: | alkaline ceramidase 2; haCER2; alkCDase 2; alkaline CDase 2; ceramide hydrolase |
| UniProt Protein Name: | Alkaline ceramidase 2 |
| UniProt Synonym Protein Names: | Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like |
| Protein Family: | Alkaline ceramidase |
| UniProt Gene Name: | ACER2Â Â |
| UniProt Entry Name: | ACER2_HUMAN |
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