The ACER1 Polyclonal Antibody (PACO07204) is a powerful tool for researchers studying ACER1, an enzyme involved in ceramide metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ACER1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in lipid signaling pathways and related fields.ACER1, also known as alkaline ceramidase 1, plays a crucial role in regulating cellular ceramide levels, which are key to multiple cellular processes including apoptosis, cell growth, and inflammation.
Dysregulation of ceramide metabolism has been linked to various diseases, making ACER1 a promising target for drug development and therapeutic interventions. By understanding the function of ACER1, researchers can uncover new insights into conditions such as cancer, metabolic disorders, and neurodegenerative diseases, paving the way for innovative treatment strategies.
Antibody Name:
ACER1 Antibody (PACO07204)
Antibody SKU:
PACO07204
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:10000, WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from the Internal region of human ASAH3. at AA rangle: 100-180
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of HepG2 using ASAH3 antibody. Secondary antibody was diluted at 1:20000.
ACER1: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids. Belongs to the alkaline ceramidase family.Protein type: Hydrolase; Membrane protein, multi-pass; EC 3.5.1.23; Membrane protein, integral; Lipid Metabolism - sphingolipid; Endoplasmic reticulumChromosomal Location of Human Ortholog: 19p13.3Cellular Component: endoplasmic reticulum; endoplasmic reticulum membraneMolecular Function: ceramidase activityBiological Process: cell differentiation; ceramide catabolic process; epidermis development; keratinocyte differentiation; response to alkalinity; sphingolipid biosynthetic process; sphingolipid metabolic process; sphingosine biosynthetic process
UniProt Protein Details:
NCBI Summary:
Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010]
