The ACE Antibody (PAC062663) offered by Assay Genie is a high-quality polyclonal antibody designed for research involving the ACE protein. ACE, or angiotensin-converting enzyme, is a key regulator of the renin-angiotensin system and plays a critical role in blood pressure regulation and cardiovascular function. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blotting. It specifically binds to the ACE protein, allowing for accurate detection and analysis in a variety of cell types.
This makes it an ideal tool for studies in cardiovascular biology, hypertension research, and drug development targeting the renin-angiotensin system.The ACE Antibody from Assay Genie provides researchers with a reliable and efficient tool for investigating the role of ACE in health and disease. By understanding the function and regulation of ACE, researchers can gain insights into cardiovascular disorders, kidney diseases, and other conditions influenced by the renin-angiotensin system. Stay ahead in your research with the ACE Antibody from Assay Genie.
Antibody Name:
Ace Antibody (PACO62663)
Antibody SKU:
PACO62663
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Rat
Immunogen:
Recombinant Rat Angiotensin-converting enzyme protein (1046-1263AA)
IHC image of PACO62663 diluted at 1:400 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm (By similarity).
ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Membrane protein, integral; EC 3.4.15.1; Protease
Cellular Component: basal plasma membrane; brush border membrane; cytoplasm; endosome; external side of plasma membrane; extracellular space; integral to membrane; lysosome; plasma membrane; vesicle
Molecular Function:actin binding; bradykinin receptor binding; carboxypeptidase activity; chloride ion binding; drug binding; endopeptidase activity; exopeptidase activity; metal ion binding; metallopeptidase activity; mitogen-activated protein kinase binding; mitogen-activated protein kinase kinase binding; peptidase activity; peptidyl-dipeptidase activity; tripeptidyl-peptidase activity; zinc ion binding
Biological Process: aging; alveolus development; arachidonic acid secretion; beta-amyloid metabolic process; brain development; eating behavior; female pregnancy; heart contraction; hormone catabolic process; kidney development; lung development; male gonad development; negative regulation of protein binding; neutrophil mediated immunity; organ regeneration; peptide catabolic process; peptide metabolic process; positive regulation of apoptosis; positive regulation of inflammatory response; positive regulation of neurogenesis; positive regulation of protein binding; positive regulation of systemic arterial blood pressure; proteolysis; regulation of angiotensin metabolic process; regulation of blood pressure; regulation of smooth muscle cell migration; regulation of systemic arterial blood pressure by renin-angiotensin; response to drug; response to hypoxia; response to lipopolysaccharide; response to nutrient levels; sensory perception of pain; spermatogenesis; vasoconstriction
NCBI Summary:
catalyzes the conversion of angiotensin I to angiotensin II; plays a role in regulation of blood pressure [RGD, Feb 2006]
