The ACAT1 Polyclonal Antibody (CAB5335) is a valuable tool for researchers studying ACAT1, an enzyme involved in cholesterol metabolism and lipid storage. This antibody, generated in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the ACAT1 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in metabolic disorders, cardiovascular diseases, and lipid metabolism research.
ACAT1, also known as acetyl-CoA acetyltransferase 1, plays a crucial role in the regulation of cellular cholesterol levels and the synthesis of cholesterol esters. Dysregulation of ACAT1 activity has been implicated in a range of health conditions, including atherosclerosis, obesity, and metabolic syndrome. Understanding the function of ACAT1 is essential for developing therapeutic strategies aimed at addressing these diseases and improving overall health outcomes.
Product Name:
ACAT1 Polyclonal Antibody
SKU:
CAB5335
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Purification Method:
Affinity purification
Gene ID:
38
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ACAT1 Rabbit pAb (CAB5335) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
