The ACAT1 Polyclonal Antibody (CAB13273) is a powerful tool for researchers studying ACAT1, a key enzyme involved in cholesterol metabolism. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ACAT1 protein, this antibody allows for the detection and analysis of ACAT1 levels in various cell types, making it an excellent choice for studies in biochemistry and lipid metabolism research.ACAT1, also known as acyl-CoA:cholesterol acyltransferase 1, plays a crucial role in the esterification of cholesterol, a process essential for the storage and transport of this lipid in cells.
Dysregulation of ACAT1 activity has been implicated in various diseases, including atherosclerosis and metabolic disorders. By understanding the function of ACAT1, researchers may uncover potential therapeutic targets for these conditions.Overall, the ACAT1 Polyclonal Antibody (CAB13273) offers a reliable tool for investigating the role of ACAT1 in cholesterol metabolism and related diseases, providing valuable insights for drug development and clinical management.
Product Name:
ACAT1 Rabbit Polyclonal Antibody
SKU:
CAB13273
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Purification Method:
Affinity purification
Gene ID:
38
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ACAT1 Rabbit pAb (CAB13273) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
