The ACAN Polyclonal Antibody (CAB12045) is a vital tool for research involving ACAN, a key protein involved in cartilage development and maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to the ACAN protein, this antibody enables accurate detection and analysis in a range of cell types, making it ideal for studies in orthopedics, musculoskeletal biology, and regenerative medicine.ACAN, also known as aggrecan, is a major component of the extracellular matrix in cartilage tissues, where it plays a crucial role in maintaining cartilage structure and function.
Dysregulation of ACAN expression and function has been linked to various musculoskeletal disorders, such as osteoarthritis and intervertebral disc degeneration. Understanding the role of ACAN in cartilage biology is essential for developing new therapeutic strategies to treat these debilitating conditions.In summary, the ACAN Polyclonal Antibody (CAB12045) offers researchers a valuable tool for studying the function and regulation of ACAN in cartilage biology and related fields. Its high specificity and sensitivity make it a reliable choice for investigating the mechanisms underlying cartilage development, maintenance, and pathologies.
Product Name:
Aggrecan Rabbit Polyclonal Antibody
SKU:
CAB12045
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 420-575 of human Aggrecan (NP_037359.3).
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
Purification Method:
Affinity purification
Gene ID:
176
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse brain, using Aggrecan antibody (CAB12045) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
