Based on the information in the provided URL, the ABLIM1 Antibody (PAC015241) is a valuable tool for research involving ABLIM1, a protein involved in actin dynamics and cell migration. This polyclonal antibody, generated in rabbits, is highly specific for human samples and has been validated for use in various experimental techniques, including immunofluorescence and immunohistochemistry.ABLIM1 is known to play a crucial role in cell adhesion and motility, making it a key player in processes such as tissue development and wound healing. Dysregulation of ABLIM1 has been linked to diseases like cancer and neurological disorders, highlighting its importance in cellular function and disease pathology.
By using the ABLIM1 Antibody, researchers can investigate the expression and localization of ABLIM1 in different cell types and tissues, shedding light on its functions and potential therapeutic implications.Overall, the ABLIM1 Antibody (PAC015241) offers a reliable tool for studying the role of ABLIM1 in cell biology and disease research, providing valuable insights into the molecular mechanisms underlying various pathologies. Its specificity and versatility make it a valuable asset for scientists working in the fields of cancer biology, neuroscience, and developmental biology.
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse heart tissue, Primary antibody: PACO15241(ABLIM1 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO15241(ABLIM1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a cytoskeletal LIM protein that binds to actin filaments via a domain that is homologous to erythrocyte dematin. LIM domains, found in over 60 proteins, play key roles in the regulation of developmental pathways. LIM domains also function as protein-binding interfaces, mediating specific protein-protein interactions. The protein encoded by this gene could mediate such interactions between actin filaments and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified.
Synonyms:
Actin binding LIM protein 1
UniProt Protein Function:
abLIM: a cytoskeletal LIM protein consisting of a C-terminal cytoskeletal domain fused to an N-terminal domain of four double zinc finger motifs. The C-terminal domain is 50% identical to dematin, an actin-bundling protein of the erythroid cytoskeleton. Undergoes extensive phosphorylation in light-adapted retinas in vivo and its developmental expression in the retina coincides with the elaboration of photoreceptor inner and outer segments. Three distinct splice variant isoforms have been described: ABLIM-l, ABLIM-m, ABLIM-s.Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-bindingChromosomal Location of Human Ortholog: 10q25Cellular Component: lamellipodium; cytoplasm; stress fiber; actin cytoskeletonMolecular Function: protein binding; zinc ion binding; actin bindingBiological Process: lamellipodium biogenesis; axon guidance; organ morphogenesis; visual perception; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; cytoskeleton organization and biogenesis
UniProt Protein Details:
NCBI Summary:
This gene encodes a cytoskeletal LIM protein that binds to actin filaments via a domain that is homologous to erythrocyte dematin. LIM domains, found in over 60 proteins, play key roles in the regulation of developmental pathways. LIM domains also function as protein-binding interfaces, mediating specific protein-protein interactions. The protein encoded by this gene could mediate such interactions between actin filaments and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
